BactoBox EasyCount measures the total bacterial cell count by default, but it can be customized, in most cases, to differentiate between viable and non-viable bacteria based on cell membrane integrity. In SBT Instruments' experience with over 200 operational original BactoBox units, it has been found that in liquid cultures—such as overnight or actively growing cultures—typically more than 90% of the cells are viable, making live/dead differentiation unnecessary. Omitting the live/dead customization significantly reduces setup time and simplifies operation. Configuration for live/dead differentiation is available for a fee, as it requires setup on a species-level basis.

BactoBox EasyCount offers significant improvements over OD600 by providing a precise and accurate total bacterial cell count, rather than just an optical density measurement. OD600 is an indirect method that estimates cell density based on turbidity, which can be affected by factors such as media composition, pigments, and cell size, often requiring calibration curves for different strains and conditions. In contrast, BactoBox EasyCount directly counts the bacterial cells, delivering consistent and reliable results that are not influenced by these variables. This eliminates the need for calibration, saves time, and provides a more accurate representation of the actual bacterial concentration in your culture.

Yes, BactoBox EasyCount is well-suited for use with strictly anaerobic bacteria. BactoBox EasyCount exhibits consistent efficiency in enumerating both anaerobic and aerobic bacteria, reflecting its adaptability across a diverse range of microbial strains. Its design features, such as compact size and ease of handling, make it an ideal choice for working in anaerobic conditions. The BactoBox EasyCount device, along with the necessary diluents, can be conveniently placed inside an anaerobic chamber. 

The standard sample volume used for BactoBox EasyCount is approximately 100µL, which is used to make a 1:10,000 dilution before measuring.